16751776 Dataset Description
Display information related to the dataset used on the piRNA search processing and stored on piRNAdb. Basic statistics, like access code and piRNAs found in this dataset are available. Also information about the title, authors and abstract of the published paper. In the end is provided a list of all piRNAs, and their links, that were found in this dataset.
To get more information about how we select the datasets that will be part of piRNAdb, access the specific page "About", item: Dataset Selection Criteria
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- Dataset Code
-
16751776
- Serie
- Organism
- Rattus norvegicus
- Method
- Immunoprecipitation
- Tissue
- testis
- Quantity piRNA
-
18950
16751776 Pubmed Informations
Retrieved from Pubmed website
- Title
- A germline-specific class of small RNAs binds mammalian Piwi proteins.
- Publication Date
- 08/09/2006
- Abstract
- Small RNAs associate with Argonaute proteins and serve as sequence-specific guides to regulate messenger RNA stability, protein synthesis, chromatin organization and genome structure. In animals, Argonaute proteins segregate into two subfamilies. The Argonaute subfamily acts in RNA interference and in microRNA-mediated gene regulation using 21-22-nucleotide RNAs as guides. The Piwi subfamily is involved in germline-specific events such as germline stem cell maintenance and meiosis. However, neither the biochemical function of Piwi proteins nor the nature of their small RNA guides is known. Here we show that MIWI, a murine Piwi protein, binds a previously uncharacterized class of approximately 29-30-nucleotide RNAs that are highly abundant in testes. We have therefore named these Piwi-interacting RNAs (piRNAs). piRNAs show distinctive localization patterns in the genome, being predominantly grouped into 20-90-kilobase clusters, wherein long stretches of small RNAs are derived from only one strand. Similar piRNAs are also found in human and rat, with major clusters occurring in syntenic locations. Although their function must still be resolved, the abundance of piRNAs in germline cells and the male sterility of Miwi mutants suggest a role in gametogenesis.
- Author(s)
- Girard, Angélique; Sachidanandam, Ravi; Hannon, Gregory J; Carmell, Michelle A;