16778019 Dataset Description
Display information related to the dataset used on the piRNA search processing and stored on piRNAdb. Basic statistics, like access code and piRNAs found in this dataset are available. Also information about the title, authors and abstract of the published paper. In the end is provided a list of all piRNAs, and their links, that were found in this dataset.
To get more information about how we select the datasets that will be part of piRNAdb, access the specific page "About", item: Dataset Selection Criteria
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- Dataset Code
-
16778019
- Serie
- Organism
- Rattus norvegicus
- Method
- small RNA-seq
- Tissue
- testis
- Quantity piRNA
-
39507
16778019 Pubmed Informations
Retrieved from Pubmed website
- Title
- Characterization of the piRNA complex from rat testes.
- Publication Date
- 07/31/2006
- Abstract
- Small noncoding RNAs regulate processes essential for cell growth and development, including mRNA degradation, translational repression, and transcriptional gene silencing (TGS). During a search for candidate mammalian factors for TGS, we purified a complex that contains small RNAs and Riwi, the rat homolog to human Piwi. The RNAs, frequently 29 to 30 nucleotides in length, are called Piwi-interacting RNAs (piRNAs), 94% of which map to 100 defined (< or = 101 kb) genomic regions. Within these regions, the piRNAs generally distribute across only one genomic strand or distribute on two strands but in a divergent, nonoverlapping manner. Preparations of piRNA complex (piRC) contain rRecQ1, which is homologous to qde-3 from Neurospora, a gene implicated in silencing pathways. Piwi has been genetically linked to TGS in flies, and slicer activity cofractionates with the purified complex. These results are consistent with a gene-silencing role for piRC in mammals.
- Author(s)
- Lau, Nelson C; Seto, Anita G; Kim, Jinkuk; Kuramochi-Miyagawa, Satomi; Nakano, Toru; Bartel, David P; Kingston, Robert E;