27429044 Dataset Description

Display information related to the dataset used on the piRNA search processing and stored on piRNAdb. Basic statistics, like access code and piRNAs found in this dataset are available. Also information about the title, authors and abstract of the published paper. In the end is provided a list of all piRNAs, and their links, that were found in this dataset.
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• Dataset Code
• 27429044
• Serie
• Organism
• Homo sapiens
• Method
• small RNA-seq
• Tissue
• liver
• Quantity piRNA
• 153

27429044 Pubmed Informations
Retrieved from Pubmed website

• Title
• Specific patterns of PIWI-interacting small noncoding RNA expression in dysplastic liver nodules and hepatocellular carcinoma.
• Publication Date
• 01/29/2018
• Abstract
• Hepatocellular carcinoma (HCC) is the result of a stepwise process, often beginning with development within a cirrhotic liver of premalignant lesions, morphologically characterized by low- (LGDN) and high-grade (HGDN) dysplastic nodules. PIWI-interacting RNAs (piRNAs) are small noncoding RNAs (sncRNAs), 23-35 nucleotide-long, exerting epigenetic and post-transcriptional regulation of gene expression. Recently the PIWI-piRNA pathway, best characterized in germline cells, has been identified also in somatic tissues, including stem and cancer cells, where it influences key cellular processes.Small RNA sequencing was applied to search for liver piRNAs and to profile their expression patterns in cirrhotic nodules (CNs), LGDN, HGDN, early HCC and progressed HCC (pHCC), analyzing 55 samples (14 CN, 9 LGDN, 6 HGDN, 6 eHCC and 20 pHCC) from 17 patients, aiming at identifying possible relationships between these sncRNAs and liver carcinogenesis. We identified a 125 piRNA expression signature that characterize HCC from matched CNs, correlating also to microvascular invasion in HCC. Functional analysis of the predicted RNA targets of deregulated piRNAs indicates that these can target key signaling pathways involved in hepatocarcinogenesis and HCC progression, thereby affecting their activity. Interestingly, 24 piRNAs showed specific expression patterns in dysplastic nodules, respect to cirrhotic liver and/or pHCC.The results demonstrate that the PIWI-piRNA pathway is active in human liver, where it represents a new player in the molecular events that characterize hepatocarcinogenesis, from early stages to pHCC. Furthermore, they suggest that piRNAs might be new disease biomarkers, useful for differential diagnosis of dysplastic and neoplastic liver lesions.
• Author(s)
• Rizzo, Francesca; Rinaldi, Antonio; Marchese, Giovanna; Coviello, Elena; Sellitto, Assunta; Cordella, Angela; Giurato, Giorgio; Nassa, Giovanni; Ravo, Maria; Tarallo, Roberta; Milanesi, Luciano; Destro, Anna; Torzilli, Guido; Roncalli, Massimo; Di Tommaso, Luca; Weisz, Alessandro;